The present study was carried out to develop Mucoadhesive drug delivery System in the form of microsphere dosage form of Nifedipine by using Carbopol and RLPO and thereafter formulating the formulation. From the study it was observed that formulation act as prolonged dosage form. As the stirring speed increased the size of microsphere decreases and increases the released rate drug. The prepared microsphere of Nifedipine also gave good Micrometrics result, percent yield, drug entrapment and in-vitro release. In dissolution study of all formulations it was observed that change in process variables during the formulation of microspheres like stirring speed (RPM) and stirring time significantly affect the release rate of drug. The microspheres of F7 batch were found to be satisfactory in terms of percent yield, percent drug entrapment and in-vitro release; Surface morphology by stereomicroscope gives smooth surface of all batches.