Zero Absorbance UV Spectrophotometric Assay Method for Simultaneous Determination of Amlodipine Besylate and Valsartan

Rahul R. Nahire, Sagar S. JoshI, Nalini Shastri, K. V. Surendra Nath

Abstract


Simple and rapid assay method is primary prerequisite in quality assurance department for any pharmaceutical dosage form.
When two drugs are combined in a single dosage form, it becomes difficult to estimate them simultaneously with required
accuracy and precision. Here we have proposed a simple and rapid UV spectrophotometric assay method for simultaneous
determination of amlodipine besylate and valsartan in both bulk drug and commercial dosage forms. Method employed was
Zero absorbance method where amlodipine and valsartan were determined at 363 nm and 290 nm respectively. At 363 nm
valsartan don’t have absorption and at 290 nm amlodipine has zero absorption. Linearity was established at 8 μg/mL – 80
μg/mL for amlodipine (y= 0.012X + 0.002 R2= 0.999) and 4 μg/mL – 300 μg/ml for Vasartan (y= 0.002X + 0.017 R2= 0.999).
Method was found to be accurate and precise with %RSD<2 for all the validation parameters employed. Method was found to
be very sensitive with LOD 2.4 μg/mL and 3.6 μg/mL whereas LOQ was 7.5 μg/mL and 11 μg/mL for Amlodipine besylate and
Valsartan respectively. As method uses methanol as a solvent to dissolve, it is cost effective for routine analysis in quality
assurance department for determination of these drugs individually or simultaneously.

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